Cloning, Transformation and Expression of Gene Clusters

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Conjugation using ET12567/pUZ8002

Heterologous expression of gene clusters

PAC cloning

Protoplasts Formation, Regeneration and Transformation

TAR cloning

Tri-parental mating to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into Streptomyces.

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