Consumables for Southern Blotting
From ActinoBase
Roche provides a DIG DNA Labelling and Detection Kit that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme and NBT/BCIP solution. Otherwise, the components can be purchased separately.
Item | Recommended Supplier | Usage | Storage | |
---|---|---|---|---|
General | Hybond N+ membrane (rolls) | GE Healthcare | ̴ 20 blots | RT |
Whatman paper | VWR or Fisher | ̴ 100 blots | RT | |
Blocking reagent | Roche | ̴ 25 blots | 4°C | |
Anti-DIG-AP conjugate | Roche | ̴ 60 reactions | -20°C | |
To label probe | Either: Hexanucleotide Mix + DIG DNA Labelling Mix +Klenow Enzyme | Roche | 25 to 50 reactions | -20°C |
Or: PCR DIG labelling mix + PCR components | Roche | 100 reactions | -20°C | |
For detection | Either: NBT/BCIP tablets | Roche | 10-20 blots | 4°C |
Or: NBT/BCIP solution | Roche | -20°C |
Also required:
- Ammonium acetate
- EDTA
- HCl concetrated
- Maleic acid
- N-laurylsarcosine
- NaCl
- Sodium Citrate
- SDS
- NaOH
- Tris Base
- Tween-20