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=Difco Nutrient Agar (DNA)=
=Difco Nutrient Agar (DNA)=
Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.
Many Streptomyces sp. grow well on DNA, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. [[SFM]]).


==Preparation==
==Preparation==
For 1 Litre:
Per Litre:
*4g Difco Nutrient Broth Powder
*4g Difco Nutrient Broth Powder
*10g Agar
*10g Agar
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*[[Spore titres]]
*[[Spore titres]]
*[[Re-isolation experiments]]
*[[Re-isolation experiments]]
 
*[[Selection for resistance to salt-sensitive antibiotics]]


==Notes==
==Notes==
For growth in liquid culture, [[Difco Nutrient Broth]] can be made without the agar.


==References==
==References==

Revision as of 14:28, 25 June 2019

Difco Nutrient Agar (DNA)

Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.

Many Streptomyces sp. grow well on DNA, but developmental and/or antibiotic production phenotypes may differ compared to growth on other media (e.g. SFM).

Preparation

Per Litre:

  • 4g Difco Nutrient Broth Powder
  • 10g Agar
  • 1L dH2O

Instructions

  1. For solid medium, add 0.8g of DNB powder into each 250ml conical flask
  2. Add 2g of Agar
  3. Add 200ml dH2O
  4. Autoclave


Uses

Notes

For growth in liquid culture, Difco Nutrient Broth can be made without the agar.

References