DNB: Difference between revisions
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=Difco Nutrient | =Difco Nutrient Broth (DNB)= | ||
Difco Nutrient | Difco Nutrient Broth is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches. | ||
==Preparation== | ==Preparation== | ||
Per Litre: | Per Litre: | ||
*4g Difco Nutrient Broth Powder | *4g Difco Nutrient Broth Powder | ||
*1L dH2O | *1L dH2O | ||
'''Instructions''' | '''Instructions''' | ||
# | #Add DNB powder to dH2O and mix to dissolve | ||
#Autoclave | #Autoclave | ||
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==Notes== | ==Notes== | ||
According to the recipe in Practical Streptomyces Genetics, it should be 8 g DNB per litre. | |||
==References== | ==References== | ||
Revision as of 15:02, 25 June 2019
Difco Nutrient Broth (DNB)
Difco Nutrient Broth is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.
Preparation
Per Litre:
- 4g Difco Nutrient Broth Powder
- 1L dH2O
Instructions
- Add DNB powder to dH2O and mix to dissolve
- Autoclave
Uses
- Streptomyces Colony PCR
- Spore titres
- Re-isolation experiments
- Selection for resistance to salt-sensitive antibiotics
Notes
According to the recipe in Practical Streptomyces Genetics, it should be 8 g DNB per litre.
