DNB: Difference between revisions
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==Uses== | ==Uses== | ||
*[[Selection for resistance to salt-sensitive antibiotics]] | *[[Selection for resistance to salt-sensitive antibiotics]] | ||
==Notes== | ==Notes== | ||
According to the recipe in Practical Streptomyces Genetics, it should be 8 g DNB per litre | According to the recipe in Practical Streptomyces Genetics, it should be 8 g DNB per litre (can someone check this please and edit the recipe with the correct amount if necessary) | ||
==References== | ==References== | ||
Revision as of 15:04, 25 June 2019
Difco Nutrient Broth (DNB)
Difco Nutrient Broth is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.
Preparation
Per Litre:
- 4g Difco Nutrient Broth Powder
- 1L dH2O
Instructions
- Add DNB powder to dH2O and mix to dissolve
- Autoclave
Uses
Notes
According to the recipe in Practical Streptomyces Genetics, it should be 8 g DNB per litre (can someone check this please and edit the recipe with the correct amount if necessary)
