DNB

Difco Nutrient Broth (DNB)

Difco Nutrient Broth is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains beef extract and peptones; exact composition will vary between batches.

Preparation

Per Litre:

• 4g Difco Nutrient Broth Powder
• 1L dH2O

Instructions

1. Add DNB powder to dH2O and mix to dissolve
2. Autoclave

Uses

• Streptomyces Colony PCR
• Spore titres
• Re-isolation experiments
• Selection for resistance to salt-sensitive antibiotics

Notes

According to the recipe in Practical Streptomyces Genetics, it should be 8 g DNB per litre.