HV

HV

Preparation

Per Litre:

• 1g humic acid
• 1.7g KCl
• 0.5g Na₂HPO₄
• 0.5g MgSO₄
• 0.02g CaCO₃
• 0.01g FeSO₄
• 1mL VB stock solution (added after autoclaving)
• 10g agar
• 1L dH₂O

Instructions

1. Combine all components (except for the VB stock solution) and mix well.
2. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
3. Add 1ml VB stock solution, mix well and pour into sterile Petri dishes.

Uses

• Isolation experiments

Notes

VB stock solution: 50mg VB1 (thiamine), VB2 (riboflavin), VB3 (niacin), VB6 (pyridoxine), B5 (D-calcium pantothenate), inositol and PABA (para amino acid), 25mg B7 (Biotin),and 100mL distilled water).

References

Hayakawa and Nonomura (1987). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. Journal of Fermentation Technology 65(5): 501-509.