Indicator compounds

Indicator Compounds

5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)

Uses

• Detection of β-galactosidase activity on solid media
• Bacterial two-hybrid analysis^[1]

Instructions for use

5-Bromo-4-chloro-1'H'-indol-3-yl β-D-glucopyranosiduronic acid (X-Gluc)

Uses

• Detection of β-glucuronidase activity on solid media
• Screening for β-glucuronidase -positive or -negative candidates^[1]

Instructions for use

1. C

References

[1] Myronovskyi, M., Welle, E., Fedorenko, V., & Luzhetskyy, A. (2011). Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes. Applied and environmental microbiology, 77(15), 5370–5383. https://doi.org/10.1128/AEM.00434-11