Lambda DNA/HindIII Ladder
From ActinoBase
Lambda HindIII Marker Preparation
- Lambda (λ) DNA (500 µg/ml): 100µl
- 10x Reaction Buffer: 45 µl
- HindIII or PstI(10U/µl): 5µl
- ddWater: 295µl
Preparation:
- Incubate 90 minutes 37°C
- Add 5µl HindIII or PstI
- Incubate 90 minutes 37°
- Incubate 10 minutes, 75°C
- Store at –20°C
Add 6x loading dye before loading into gel
Size and Concentration of Bands
| Size of Bands in bp (λ HindIII) | ng in 5µl |
|---|---|
| (27491) | - |
| (23130) | 240 |
| 9416 | 97 |
| 6556 | 68 |
| (4361) | 44 |
| 2322 | 24 |
| 2027 | 21 |
| 561 | 6 |
| 125 | - |
