MM

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MM Medium

Preparation

Per litre:

  • 0.5g L-asparagine
  • 0.5g K2HPO4
  • 0.2g MgSO4*7H2O
  • 0.01g FeSO4*7H2O
  • 10g glucose (added after autoclaving)
  • 10g agar
  • =1L dH2O

Instructions

  1. Dissolve all of the ingredients except the agar and the glucose in the distilled water
  2. Adjust the pH to 7.0-7.2
  3. Dispense 200ml into flasks each containing 2g agar
  4. Autoclave
  5. After autoclaving, add 4 ml sterile 50% glucose solution to each flask
  6. Add any other growth factors required for auxotrophic mutants

Uses

Notes

References

Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.