MYM: Difference between revisions
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=MYM Medium= | =MYM Medium= | ||
Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of ''[[Streptomyces venezuelae]]'', as it sporulates to >90% completion in liquid MYM (Bibb et al 2012). Other <em>Streptomyces</em> sp. may also grow well on this medium. | |||
==Preparation== | |||
Per litre: | |||
*4g Maltose | *4g Maltose | ||
*4g Yeast Extract Oxoid | *4g Yeast Extract Oxoid | ||
*10g Malt Extract | *10g Malt Extract | ||
*500ml Tap Water | *500ml Tap Water | ||
*500ml dH2O | *500ml dH2O | ||
'''Instructions''' | '''Instructions''' | ||
| Line 24: | Line 18: | ||
#For solid medium add add 2g agar & 100mL liquid MYM to each flask | #For solid medium add add 2g agar & 100mL liquid MYM to each flask | ||
#Autoclave, expect a precipitate to form afterward. | #Autoclave, expect a precipitate to form afterward. | ||
# | #For optimal ''[[Streptomyces venezuelae]]'' sporulation, add [[trace elements]] at a 1:500 dilution. | ||
==Uses== | |||
*routine growth of ''[[Streptomyces venezuelae]]'' | |||
*developmental studies on ''[[Streptomyces venezuelae]]'' | |||
==Notes== | |||
If using for growth measurements in liquid medium, it is best to allow the precipitate to settle overnight before pipetting the medium into growth vessels - take from the top to avoid the precipitate. | |||
==References== | |||
Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121. | |||
Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049 | |||
Revision as of 15:39, 25 June 2019
MYM Medium
Maltose-Yeast Extract-Malt Extract (MYM) medium is a rich medium used especially for the growth of Streptomyces venezuelae, as it sporulates to >90% completion in liquid MYM (Bibb et al 2012). Other Streptomyces sp. may also grow well on this medium.
Preparation
Per litre:
- 4g Maltose
- 4g Yeast Extract Oxoid
- 10g Malt Extract
- 500ml Tap Water
- 500ml dH2O
Instructions
- Dissolve all ingredients in 500ml tap water & 250ml dH2O
- pH to 7.2-7.3 with NaOH
- add dH2O up to 1L
- For solid medium add add 2g agar & 100mL liquid MYM to each flask
- Autoclave, expect a precipitate to form afterward.
- For optimal Streptomyces venezuelae sporulation, add trace elements at a 1:500 dilution.
Uses
- routine growth of Streptomyces venezuelae
- developmental studies on Streptomyces venezuelae
Notes
If using for growth measurements in liquid medium, it is best to allow the precipitate to settle overnight before pipetting the medium into growth vessels - take from the top to avoid the precipitate.
References
Stuttard C. 1982. Temperate phages of Streptomyces venezuelae: lysogeny and host specificity shown by phages SV1 and SV2. Microbiology 128:115–121.
Bibb MJ, Domonkos A, Chandra G, Buttner MJ. 2012. Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN. Mol. Microbiol. 84:1033–1049
