Difference between revisions of "Media recipes"

From ActinoBase
Line 22: Line 22:
  
 
[[IMA]] (Smash)
 
[[IMA]] (Smash)
 
<u>ISP = International <em>Streptomyces</em> Project media:</u>
 
 
[[ISP2]]
 
 
[[ISP3]]
 
 
[[ISP4]]
 
 
[[ISP5]]
 
 
[[ISP6]]
 
 
[[ISP7]]
 
  
 
[[LB]] (Lysogeny Broth) | [[L]] (L agar)
 
[[LB]] (Lysogeny Broth) | [[L]] (L agar)
Line 60: Line 46:
  
 
[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)
 
[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)
 +
 +
<u>ISP = International <em>Streptomyces</em> Project media:</u>
 +
 +
[[ISP2]]
 +
 +
[[ISP3]]
 +
 +
[[ISP4]]
 +
 +
[[ISP5]]
 +
 +
[[ISP6]]
 +
 +
[[ISP7]]
  
 
[[SPY]] (Salt Peptone Yeast)
 
[[SPY]] (Salt Peptone Yeast)
Line 70: Line 70:
  
 
[[2X YT]]
 
[[2X YT]]
 +
 +
<u>ISP = International <em>Streptomyces</em> Project media:</u>
 +
 +
[[ISP2]]
 +
 +
[[ISP3]]
 +
 +
[[ISP4]]
 +
 +
[[ISP5]]
 +
 +
[[ISP6]]
 +
 +
[[ISP7]]
  
 
==Media Supplements==
 
==Media Supplements==

Revision as of 17:11, 8 August 2019


Media Recipes

Media Recipes

A1

CM (Complete medium)

DNA (Difco Nutrient Agar) | DNB (Difco Nutrient Broth) | SNA (Soft Nutrient Agar)

Emerson medium

Gause's No. 1

GYM (Glucose + Yeast Extract + Malt Extract)

HT (Hickey-Tresner)

HV

IMA (Smash)

LB (Lysogeny Broth) | L (L agar)

MC agar (Milk Casein Agar)

MM (Minimal medium)

MS (Mannitol Soya agar - a.k.a. Soya Flour Mannitol)

MYM (Maltose + Yeast Extract + Malt Extract)

NMMP (minimal liquid medium)

OT (oligotrophic agar)

PDA (Potato Dextrose Agar)

R2 | R2YE | R5

SC (Starch Casein agar)

SFM (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)

SMM (supplemented liquid minimal medium) | SMMS (supplemented minimal medium, solid)

ISP = International Streptomyces Project media:

ISP2

ISP3

ISP4

ISP5

ISP6

ISP7

SPY (Salt Peptone Yeast)

TSB (Tryptone Soy Broth)

YEME (Yeast Extract + Malt Extract)

YP (Yeast Peptone) | YPD (Yeast Peptone Dextrose)

2X YT

ISP = International Streptomyces Project media:

ISP2

ISP3

ISP4

ISP5

ISP6

ISP7

Media Supplements

Antibiotic stocks and working concentrations

Indicator compounds

Media supplements for activating cryptic biosynthetic gene clusters

Media supplements for induction gene expression from inducible promoters