PIJ6021

Plasmid pIJ6021 allows for high level protein production in Streptomyces species. This plasmid was first described by Takano et al 1995 and utilises the pIJ101 origin of replication which leads to high copy number, sometimes approaching 100 copies per cell. It includes the thiostrepton inducible tipA promoter to induce expression of the target gene.

pIJ6021 lacks an E. coli replicon, meaning (i) any cloning must be done using Streptomyces spp.; and (ii) plasmid DNA cannot be transferred to Streptomyces spp. by conjugal transfer from E. coli.