pIJ773

Use

pIJ773 is a plasmid used during PCR targeting for mutagenesis experiments in actinomycetes. The plasmid is used as a template to amplify a disruption cassette by PCR, producing a 1382 bp fragment.

Features

• Apramycin resistance (aac(3)IV)
• oriT from RP4 plasmid (RK2)
• Flippase recognition target (FRT) sites

References

1. Gust, B., Challis, G. L., Fowler, K., Kieser, T., Chater, K. F. (2003) PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin Proceedings of the National Academy of Sciences of the United States of America. 100(4): 1541–1546.