pMS82

From ActinoBase
Revision as of 13:32, 25 October 2019 by Jordanmurray (talk | contribs) (→‎Use)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Use

This integration vector allows for conjugal transfer of DNA into the φBT1 phage attachment site of Streptomyces genomes from E. coli. This vector is derived from the pSET152 backbone. It contains the oriT from Streptomyces as well as the hygromycin resistance cassette.

This vector can be manipulated to contain genes of interest for the expression of homologous proteins, photoproteins, natural products etc.

Features

  • Hygromycin resistance cassette
  • φBT1 integration site & integrase

History

Map

PMS82.png


Sequence links

References

Needs formatting!!

here