pSS170

From ActinoBase
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.


Use

pSS170 is used for introducing genes to Streptomyces. Because this plasmid is lacking a promoter upstream of the multiple cloning site (MCS), the gene of interest is often cloned in with its native promoter, in order to complement a knockout mutant

Features

Streptomyces phage phiBT1 integration site. Hygromycin resistance gene, oriT, ColEi origin of replication

History

Derived from pMS82 - https://www.addgene.org/68110/

Map

Sequence links

References

pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323.