Plasmids: Difference between revisions
From ActinoBase
Yvonne Mast (talk | contribs) |
mNo edit summary |
||
Line 3: | Line 3: | ||
Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergenic conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]]. | Various plasmids have been developed for use in <em>Streptomyces</em> (most will also contain an origin of replication that functions in <em>Escherichia coli</em>, for cloning, general maintenance, and [[intergenic conjugation into Streptomyces]]). Many of the plasmids commonly used for <em>Streptomyces</em> work are '''integrative''': they insert into a specific site on the <em>Streptomyces</em> chromosome using a [[site-specific phage recombinase]]. | ||
The following is an alphabetical list of a number of useful vectors - see also [[ | The following is an alphabetical list of a number of useful vectors - see also [[Overview of Streptomyces vectors]]. | ||
==Plasmids used in <em>Streptomyces</em>== | ==Plasmids used in <em>Streptomyces</em>== | ||
Line 40: | Line 40: | ||
[[pGM190]] | [[pGM190]] | ||
==Plasmids used for PCR targeting (Redirect)== | ==Plasmids used for PCR targeting (Redirect)== | ||
[[pIJ773]] | [[pIJ773]] | ||
Revision as of 15:09, 1 August 2019
Various plasmids have been developed for use in Streptomyces (most will also contain an origin of replication that functions in Escherichia coli, for cloning, general maintenance, and intergenic conjugation into Streptomyces). Many of the plasmids commonly used for Streptomyces work are integrative: they insert into a specific site on the Streptomyces chromosome using a site-specific phage recombinase.
The following is an alphabetical list of a number of useful vectors - see also Overview of Streptomyces vectors.
Plasmids used in Streptomyces
Plasmids used for PCR targeting (Redirect)
Plasmids used for heterologous protein expression in E. coli
The following plasmids are frequently used to express proteins heterologously in E. coli, e.g. for two-hybrid analysis or protein purification.