Difference between revisions of "Protoplast Transformation"

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(Created page with "#Follow the instruction in Protoplasts Formation, Regeneration and Transformation #Add 3.85 ml P buffer to 1 g PEG sterilised. #Add 200 µl PEG-P buffer mixture and a DNA...")
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Revision as of 22:55, 24 March 2020

  1. Follow the instruction in Protoplasts Formation, Regeneration and Transformation
  2. Add 3.85 ml P buffer to 1 g PEG sterilised.
  3. Add 200 µl PEG-P buffer mixture and a DNA solution to 50 µl of the protoplast suspension.
  4. Plate it onto R5 or R2YE.
  5. Incubate the plate at 298-30ºC overnight.
  6. Overlay 1 ml sterilised water containing nalidixic acid and an appropriate antibiotic.