SMMS

SMMS (Supplemented liquid minimal media, solid)

SMM is a solid supplemented minimal medium suitable for growth of many Streptomyces sp.

Preparation

Per litre:

• 81.9 mL PEG 6000 (BDH 6.1% w/v in distilled water)
• 2.5 mL Mg₂SO₄.7H₂O - 5mM final
• 10 mL TES buffer (0.25M, pH 7.2) - 25mM final
• 1 mL NaH₂PO₄ + K₂HPO₄ (50mM each) - 1mM final
• 2 mL glucose (50% w/v)
• 1 mL Antifoam 289 (Sigma A 5551)(1% w/v)
• 0.1 mL Trace element solution
• 1 mL Difco Casaminoacids (20% w/v)
• 100 mL Total
• 25 ml/L Glycine (0.5% final)

Trace element solution: 0.1 g/L of the following:

• ZnSO₄.7H₂O
• FeSO₄.7H₂O
• MnCl₂.4H₂O
• CaCl₂.6H₂O
• NaCl

Make fresh every 2-4 weeks and filter-sterilise. Store at 4º

Instructions

1. With the exception of the trace element solution each solution must be made up and autoclaved separately
2. The solutions should be mixed in the given order and then made up to 1L with sterile distilled water
3. Addition of 10 g/L agar

References

Strauch E, Takano E, Baylts H, Bibb M. The stringent response in Streptomyces coelicolor A3(2). Molecular Microbiology. 1991;5(2):289-298.