User contributions for H Otani
From ActinoBase
18 May 2021
- 16:2616:26, 18 May 2021 diff hist +238 N PCRISPR-dCas9 Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102..." current
- 16:2516:25, 18 May 2021 diff hist +238 N PCRISPR-Cas9 Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102..." current
- 16:2316:23, 18 May 2021 diff hist +278 N PTYM18 Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector..." current
- 16:2216:22, 18 May 2021 diff hist +278 N PTYM19 Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector..." current
- 16:2216:22, 18 May 2021 diff hist +278 N PTOYAMAcos Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector..." current
- 16:1816:18, 18 May 2021 diff hist +76 Plasmids →Plasmids used in Streptomyces
15 July 2020
- 19:1319:13, 15 July 2020 diff hist −176 Recruitment →Recruitment
31 March 2020
- 05:2605:26, 31 March 2020 diff hist +23 Protoplast Transformation →Instructions current
25 March 2020
- 17:1517:15, 25 March 2020 diff hist −25 Teaching and learning →Podcasts
- 00:1800:18, 25 March 2020 diff hist +209 RNA Sequencing No edit summary
- 00:0700:07, 25 March 2020 diff hist +1,742 N RNA Sequencing Created page with "#Extract and purify RNA samples according to RNA extraction from Actinobacteria. Add [https://www.thermofisher.com/order/catalog/product/4456740#/4456740 ERCC RNA Spike-In..."
24 March 2020
- 23:4323:43, 24 March 2020 diff hist +143 N Natural Products Created page with "Cloning, Transformation and Expression of Gene Clusters Metabolite extractions Production of microbial volatile organic compounds" current
- 23:4123:41, 24 March 2020 diff hist +60 Protocols →Alphabetical list of protocols
- 23:4023:40, 24 March 2020 diff hist +60 Mutagenesis No edit summary current
- 23:3823:38, 24 March 2020 diff hist +61 Protocols →Mutagenesis
- 22:0322:03, 24 March 2020 diff hist +73 Teaching and learning →Podcasts
- 21:5821:58, 24 March 2020 diff hist −15 Protoplast Transformation →Materials
- 21:5721:57, 24 March 2020 diff hist +125 Protoplast Transformation No edit summary
- 21:5521:55, 24 March 2020 diff hist −62 Protoplasts Formation, Regeneration and Transformation →Materials current
- 21:5521:55, 24 March 2020 diff hist +390 N Protoplast Transformation Created page with "#Follow the instruction in Protoplasts Formation, Regeneration and Transformation #Add 3.85 ml P buffer to 1 g PEG sterilised. #Add 200 µl PEG-P buffer mixture and a DNA..."
- 21:5321:53, 24 March 2020 diff hist −305 Protoplasts Formation, Regeneration and Transformation →Instructions
- 21:5221:52, 24 March 2020 diff hist +26 Protoplasts Formation, Regeneration and Transformation →Materials
- 21:5221:52, 24 March 2020 diff hist +305 Protoplasts Formation, Regeneration and Transformation →Instructions
- 21:4421:44, 24 March 2020 diff hist +36 Protoplasts Formation, Regeneration and Transformation →Materials
- 21:4021:40, 24 March 2020 diff hist +67 Protoplasts Formation, Regeneration and Transformation →Instructions
- 21:3721:37, 24 March 2020 diff hist +1 Protoplasts Formation, Regeneration and Transformation →Materials
- 21:3321:33, 24 March 2020 diff hist +176 Recruitment →Recruitment
4 October 2019
- 20:5620:56, 4 October 2019 diff hist −28 Streptomyces griseus No edit summary
- 20:1220:12, 4 October 2019 diff hist +155 Groups No edit summary
3 September 2019
- 18:4618:46, 3 September 2019 diff hist +476 Rhodococcus jostii →Background current
- 18:2918:29, 3 September 2019 diff hist +1 YMPD →Preparation current
31 August 2019
- 04:3704:37, 31 August 2019 diff hist +349 N Rhodococcus jostii Created page with "==Organism Page== === ''Rhodococcus jostii'' === ===Background=== ===Growth & Maintenance=== LB or M9 =====Sporulation===== Nonsporulating ===Strains=== <em>Rh..."
- 04:3404:34, 31 August 2019 diff hist +109 m Rhodococcus No edit summary current
- 04:2304:23, 31 August 2019 diff hist +213 m Streptomyces griseus No edit summary
- 04:2004:20, 31 August 2019 diff hist +24 m YMPD No edit summary
- 00:2100:21, 31 August 2019 diff hist +684 N Rhodococcus Created page with "Rhodococci are nonsporulating actinomycetes in the family Nocardiaceae along with <em>Nocardia</em>. They are known for their ability to catabolise a wide range of organic..."
30 August 2019
- 23:5423:54, 30 August 2019 diff hist +27 Organisms Added Rhodococcus
- 23:2923:29, 30 August 2019 diff hist +369 Streptomyces griseus No edit summary
- 21:4621:46, 30 August 2019 diff hist 0 YMPD No edit summary
- 21:2221:22, 30 August 2019 diff hist +518 Streptomyces griseus No edit summary
- 20:4820:48, 30 August 2019 diff hist +516 N Saccharopolyspora spinosa Created page with "==Organism Page== === ''Saccharopolyspora spinosa'' === ===Background=== <em>Saccharopolyspora spinosa</em> is known for producing an insecticide, spinosad. Spinosad is a m..." current
- 20:4220:42, 30 August 2019 diff hist +166 Saccharopolyspora Added two species current
- 20:3720:37, 30 August 2019 diff hist +592 N YMPD Created page with "=YMPD (Yeast Meat Peptone Dextrose)= For growth of ''Streptomyces griseus''. ==Preparation== Per litre: *2 g yeast extract *2.2 g meat extract *4 g Bacto peptone *5 g Na..."
- 20:2020:20, 30 August 2019 diff hist +40 Media recipes →Media Recipes: YMPD
- 20:1320:13, 30 August 2019 diff hist +41 High GC PCR Added betaine. current
- 19:4619:46, 30 August 2019 diff hist +108 Groups Added some groups
- 19:2019:20, 30 August 2019 diff hist +90 Sequence databases Added IMG/M and Genome Portal