User contributions for MFeeney
From ActinoBase
19 July 2022
- 10:2510:25, 19 July 2022 diff hist 0 m SMM →Preparation current
- 10:2410:24, 19 July 2022 diff hist 0 m SMMS →Preparation current
20 March 2022
- 23:3423:34, 20 March 2022 diff hist +3 SV1 transduction →Notes current
- 23:3223:32, 20 March 2022 diff hist +2,428 SV1 transduction No edit summary
- 21:5021:50, 20 March 2022 diff hist +1,333 N SV1 transduction Created page with "=<strong>Phage transduction using SV1</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces venezuelae</em> with ΦSV1 ===Ph..."
- 21:4521:45, 20 March 2022 diff hist +22 Streptomyces Bacteriophages →Protocols current
21 May 2020
- 14:1114:11, 21 May 2020 diff hist +79 Protocols No edit summary
7 May 2020
- 14:3814:38, 7 May 2020 diff hist +3 Lambda-red mediated recombination using ssDNA →References current
- 14:3614:36, 7 May 2020 diff hist +120 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 14:3414:34, 7 May 2020 diff hist +57 Lambda-red mediated recombination using ssDNA →Step 9. Conjugation into Streptomyces
- 14:3414:34, 7 May 2020 diff hist +49 Lambda-red mediated recombination using ssDNA →Step 8. Transform ET12567/pUZ8002 with the mutant cosmid/plasmid.
- 14:3314:33, 7 May 2020 diff hist +50 Lambda-red mediated recombination using ssDNA →Step 6. Transform DH5a with your recombineered cosmid/plasmid
- 14:3314:33, 7 May 2020 diff hist +49 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 14:3214:32, 7 May 2020 diff hist +50 Lambda-red mediated recombination using ssDNA →Step 4. ssDNA recombineering
- 14:3214:32, 7 May 2020 diff hist +47 Lambda-red mediated recombination using ssDNA →Step 3. Transform the recombineering strain with the cosmid/plasmid
- 14:3114:31, 7 May 2020 diff hist +49 Lambda-red mediated recombination using ssDNA No edit summary
- 14:2914:29, 7 May 2020 diff hist +1,860 Lambda-red mediated recombination using ssDNA →Detailed Protocols
- 14:2114:21, 7 May 2020 diff hist +124 Lambda-red mediated recombination using ssDNA →Stage 4. Introduce mutant allele(s) into your Streptomyces strains of interest.
- 14:1814:18, 7 May 2020 diff hist +7 Lambda-red mediated recombination using ssDNA →Step 7. Confirm the sequence of your gene/DNA sequence of interest
- 14:1714:17, 7 May 2020 diff hist +5 Lambda-red mediated recombination using ssDNA →Step 4. ssDNA recombineering
- 14:1614:16, 7 May 2020 diff hist +55 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 14:1414:14, 7 May 2020 diff hist −4 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 08:5708:57, 7 May 2020 diff hist +107 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
- 08:5608:56, 7 May 2020 diff hist −35 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 08:5608:56, 7 May 2020 diff hist +94 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 08:4008:40, 7 May 2020 diff hist +103 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
- 08:3908:39, 7 May 2020 diff hist +28 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
6 May 2020
- 16:1816:18, 6 May 2020 diff hist +684 Lambda-red mediated recombination using ssDNA No edit summary
- 16:0716:07, 6 May 2020 diff hist +909 Lambda-red mediated recombination using ssDNA →Stage 4. Introduce mutant allele(s) into your Streptomyces strains of interest.
- 16:0316:03, 6 May 2020 diff hist +30 Lambda-red mediated recombination using ssDNA →Stage 3: Identify and isolate successful recombinants. Confirm correct recombination event.
- 16:0016:00, 6 May 2020 diff hist +31 Lambda-red mediated recombination using ssDNA →Materials Needed
- 16:0016:00, 6 May 2020 diff hist +94 Lambda-red mediated recombination using ssDNA →Materials Needed
- 15:5815:58, 6 May 2020 diff hist +7 Lambda-red mediated recombination using ssDNA No edit summary
- 15:5715:57, 6 May 2020 diff hist +18 Lambda-red mediated recombination using ssDNA →Lambda-red mediated recombination using ssDNA
- 15:5015:50, 6 May 2020 diff hist +1,744 Lambda-red mediated recombination using ssDNA No edit summary
- 15:3415:34, 6 May 2020 diff hist +353 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 15:3015:30, 6 May 2020 diff hist +363 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 15:2815:28, 6 May 2020 diff hist +119 Lambda-red mediated recombination using ssDNA →Step 4. ssDNA recombineering
- 15:2715:27, 6 May 2020 diff hist +319 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 15:2115:21, 6 May 2020 diff hist +792 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 15:0815:08, 6 May 2020 diff hist −3 Lambda-red mediated recombination using ssDNA →Step 3. Transform the recombineering strain with the cosmid/plasmid
- 15:0815:08, 6 May 2020 diff hist +1 Lambda-red mediated recombination using ssDNA →Step 3. Transform the recombineering strain with the cosmid/plasmid
- 15:0715:07, 6 May 2020 diff hist +394 Lambda-red mediated recombination using ssDNA →Step 3. Transform the recombineering strain with the cosmid/plasmid
- 14:5714:57, 6 May 2020 diff hist +995 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 14:2814:28, 6 May 2020 diff hist +110 Lambda-red mediated recombination using ssDNA →Detailed Protocols
- 14:2614:26, 6 May 2020 diff hist +2,061 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 14:1014:10, 6 May 2020 diff hist +117 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 13:5913:59, 6 May 2020 diff hist +943 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 13:4113:41, 6 May 2020 diff hist +1,239 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 13:3213:32, 6 May 2020 diff hist +93 Lambda-red mediated recombination using ssDNA →Detailed Protocols
