User contributions for MFeeney
From ActinoBase
19 July 2022
- 10:2510:25, 19 July 2022 diff hist 0 m SMM →Preparation current
- 10:2410:24, 19 July 2022 diff hist 0 m SMMS →Preparation current
20 March 2022
- 23:3423:34, 20 March 2022 diff hist +3 SV1 transduction →Notes current
- 23:3223:32, 20 March 2022 diff hist +2,428 SV1 transduction No edit summary
- 21:5021:50, 20 March 2022 diff hist +1,333 N SV1 transduction Created page with "=<strong>Phage transduction using SV1</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces venezuelae</em> with ΦSV1 ===Ph..."
- 21:4521:45, 20 March 2022 diff hist +22 Streptomyces Bacteriophages →Protocols current
21 May 2020
- 14:1114:11, 21 May 2020 diff hist +79 Protocols No edit summary
7 May 2020
- 14:3814:38, 7 May 2020 diff hist +3 Lambda-red mediated recombination using ssDNA →References current
- 14:3614:36, 7 May 2020 diff hist +120 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 14:3414:34, 7 May 2020 diff hist +57 Lambda-red mediated recombination using ssDNA →Step 9. Conjugation into Streptomyces
- 14:3414:34, 7 May 2020 diff hist +49 Lambda-red mediated recombination using ssDNA →Step 8. Transform ET12567/pUZ8002 with the mutant cosmid/plasmid.
- 14:3314:33, 7 May 2020 diff hist +50 Lambda-red mediated recombination using ssDNA →Step 6. Transform DH5a with your recombineered cosmid/plasmid
- 14:3314:33, 7 May 2020 diff hist +49 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 14:3214:32, 7 May 2020 diff hist +50 Lambda-red mediated recombination using ssDNA →Step 4. ssDNA recombineering
- 14:3214:32, 7 May 2020 diff hist +47 Lambda-red mediated recombination using ssDNA →Step 3. Transform the recombineering strain with the cosmid/plasmid
- 14:3114:31, 7 May 2020 diff hist +49 Lambda-red mediated recombination using ssDNA No edit summary
- 14:2914:29, 7 May 2020 diff hist +1,860 Lambda-red mediated recombination using ssDNA →Detailed Protocols
- 14:2114:21, 7 May 2020 diff hist +124 Lambda-red mediated recombination using ssDNA →Stage 4. Introduce mutant allele(s) into your Streptomyces strains of interest.
- 14:1814:18, 7 May 2020 diff hist +7 Lambda-red mediated recombination using ssDNA →Step 7. Confirm the sequence of your gene/DNA sequence of interest
- 14:1714:17, 7 May 2020 diff hist +5 Lambda-red mediated recombination using ssDNA →Step 4. ssDNA recombineering
- 14:1614:16, 7 May 2020 diff hist +55 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 14:1414:14, 7 May 2020 diff hist −4 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 08:5708:57, 7 May 2020 diff hist +107 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
- 08:5608:56, 7 May 2020 diff hist −35 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 08:5608:56, 7 May 2020 diff hist +94 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 08:4008:40, 7 May 2020 diff hist +103 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
- 08:3908:39, 7 May 2020 diff hist +28 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
6 May 2020
- 16:1816:18, 6 May 2020 diff hist +684 Lambda-red mediated recombination using ssDNA No edit summary
- 16:0716:07, 6 May 2020 diff hist +909 Lambda-red mediated recombination using ssDNA →Stage 4. Introduce mutant allele(s) into your Streptomyces strains of interest.
- 16:0316:03, 6 May 2020 diff hist +30 Lambda-red mediated recombination using ssDNA →Stage 3: Identify and isolate successful recombinants. Confirm correct recombination event.
- 16:0016:00, 6 May 2020 diff hist +31 Lambda-red mediated recombination using ssDNA →Materials Needed
- 16:0016:00, 6 May 2020 diff hist +94 Lambda-red mediated recombination using ssDNA →Materials Needed
- 15:5815:58, 6 May 2020 diff hist +7 Lambda-red mediated recombination using ssDNA No edit summary
- 15:5715:57, 6 May 2020 diff hist +18 Lambda-red mediated recombination using ssDNA →Lambda-red mediated recombination using ssDNA
- 15:5015:50, 6 May 2020 diff hist +1,744 Lambda-red mediated recombination using ssDNA No edit summary
- 15:3415:34, 6 May 2020 diff hist +353 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 15:3015:30, 6 May 2020 diff hist +363 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 15:2815:28, 6 May 2020 diff hist +119 Lambda-red mediated recombination using ssDNA →Step 4. ssDNA recombineering
- 15:2715:27, 6 May 2020 diff hist +319 Lambda-red mediated recombination using ssDNA →Step 5. Screen for successful recombinants
- 15:2115:21, 6 May 2020 diff hist +792 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 15:0815:08, 6 May 2020 diff hist −3 Lambda-red mediated recombination using ssDNA →Step 3. Transform the recombineering strain with the cosmid/plasmid
- 15:0815:08, 6 May 2020 diff hist +1 Lambda-red mediated recombination using ssDNA →Step 3. Transform the recombineering strain with the cosmid/plasmid
- 15:0715:07, 6 May 2020 diff hist +394 Lambda-red mediated recombination using ssDNA →Step 3. Transform the recombineering strain with the cosmid/plasmid
- 14:5714:57, 6 May 2020 diff hist +995 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 14:2814:28, 6 May 2020 diff hist +110 Lambda-red mediated recombination using ssDNA →Detailed Protocols
- 14:2614:26, 6 May 2020 diff hist +2,061 Lambda-red mediated recombination using ssDNA →Stage 2: ssDNA-recombineering in E. coli
- 14:1014:10, 6 May 2020 diff hist +117 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 13:5913:59, 6 May 2020 diff hist +943 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 13:4113:41, 6 May 2020 diff hist +1,239 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 13:3213:32, 6 May 2020 diff hist +93 Lambda-red mediated recombination using ssDNA →Detailed Protocols
- 13:3013:30, 6 May 2020 diff hist −2 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 13:3013:30, 6 May 2020 diff hist +773 Lambda-red mediated recombination using ssDNA →Step 2. cosmid/plasmid selection
- 11:5711:57, 6 May 2020 diff hist −1 Lambda-red mediated recombination using ssDNA →Lambda-red mediated recombination using ssDNA
- 11:5611:56, 6 May 2020 diff hist −37 Lambda-red mediated recombination using ssDNA →Uses
- 11:5611:56, 6 May 2020 diff hist +32 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:5511:55, 6 May 2020 diff hist −3 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:5011:50, 6 May 2020 diff hist +33 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:4911:49, 6 May 2020 diff hist +1 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
- 11:4311:43, 6 May 2020 diff hist +39 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:4211:42, 6 May 2020 diff hist −3 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:4111:41, 6 May 2020 diff hist +34 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
- 11:4111:41, 6 May 2020 diff hist −1 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:4011:40, 6 May 2020 diff hist +14 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:3711:37, 6 May 2020 diff hist +23 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:3511:35, 6 May 2020 diff hist +2 Lambda-red mediated recombination using ssDNA →Detailed Protocols
- 11:3411:34, 6 May 2020 diff hist +8 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:3111:31, 6 May 2020 diff hist +2 Lambda-red mediated recombination using ssDNA →Stage 1: Mutant design
- 11:3111:31, 6 May 2020 diff hist +99 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:3011:30, 6 May 2020 diff hist +51 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:2811:28, 6 May 2020 diff hist −1 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:2711:27, 6 May 2020 diff hist +1 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:2611:26, 6 May 2020 diff hist +2 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:2511:25, 6 May 2020 diff hist +748 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:1811:18, 6 May 2020 diff hist +774 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 11:1111:11, 6 May 2020 diff hist +385 Lambda-red mediated recombination using ssDNA →Stage 1: mutant design
- 11:0411:04, 6 May 2020 diff hist +61 N File:SsDNA recombineering oligos.png oligonucleotide design for ssDNA recombineering current
- 10:4810:48, 6 May 2020 diff hist +14 Lambda-red mediated recombination using ssDNA →Uses
- 10:4610:46, 6 May 2020 diff hist +893 Lambda-red mediated recombination using ssDNA No edit summary
- 10:3410:34, 6 May 2020 diff hist +442 Lambda-red mediated recombination using ssDNA →Lambda-red mediated recombination using ssDNA
- 10:2710:27, 6 May 2020 diff hist +812 Lambda-red mediated recombination using ssDNA No edit summary
- 10:1610:16, 6 May 2020 diff hist +84 N File:SsDNA recombineering protocol.png figure illustrating two key steps in the ssDNA recombineering protocol current
- 10:1410:14, 6 May 2020 diff hist +34 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 10:1310:13, 6 May 2020 diff hist +145 N File:SsDNA diagnostic pcr.png description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering current
- 10:1010:10, 6 May 2020 diff hist +21 Lambda-red mediated recombination using ssDNA →Step 1. in silico design of desired mutation(s)
- 10:0910:09, 6 May 2020 diff hist +2,231 Lambda-red mediated recombination using ssDNA →Stage 1: mutant design
5 May 2020
- 15:4315:43, 5 May 2020 diff hist +295 Lambda-red mediated recombination using ssDNA No edit summary
- 15:3515:35, 5 May 2020 diff hist −305 Lambda-red mediated recombination using ssDNA No edit summary
- 15:1515:15, 5 May 2020 diff hist −636 Lambda-red mediated recombination using ssDNA No edit summary
- 15:0815:08, 5 May 2020 diff hist +239 Lambda-red mediated recombination using ssDNA No edit summary
- 15:0415:04, 5 May 2020 diff hist +1,087 Lambda-red mediated recombination using ssDNA No edit summary
- 14:5514:55, 5 May 2020 diff hist +166 Lambda-red mediated recombination using ssDNA No edit summary
- 14:5214:52, 5 May 2020 diff hist +716 Lambda-red mediated recombination using ssDNA No edit summary
- 12:5212:52, 5 May 2020 diff hist +79 SNA No edit summary current
- 12:5112:51, 5 May 2020 diff hist +179 DNB No edit summary current
- 12:5012:50, 5 May 2020 diff hist +46 DNA No edit summary current
- 12:4012:40, 5 May 2020 diff hist +1 MediaWiki:Sidebar No edit summary
- 12:4012:40, 5 May 2020 diff hist +44 MediaWiki:Sidebar No edit summary
- 12:3112:31, 5 May 2020 diff hist +183 Protocols No edit summary
- 12:2912:29, 5 May 2020 diff hist +179 Protocols →Bacteriophage
- 11:1111:11, 5 May 2020 diff hist +219 Isolation of phages from environmental samples No edit summary