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• 15:42, 4 September 2025 MunnJ90 talk contribs created page ACT assay (Created page with "=== Example Assay Description === Equal volumes of culture supernatant and 1 M NaOH were mixed by vortexing. The samples were centrifuged at 1000 × g for 5 min, and the absorbance at 633 nm was measured. The molar extinction coefficient for actinorhodin is: :<math>\varepsilon = 15{,}135 \ \mathrm{M^{-1} \, cm^{-1}}</math> Using the Beer–Lambert law (<math>A = \varepsilon \, l \, c</math>), the concentration was calculated. The specific production yield was...")