YMPD: Difference between revisions

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*4 g Bacto peptone
*4 g Bacto peptone
*5 g NaCl
*5 g NaCl
*2 g magnesium sulphate heptahydrate (MgSO<sub>4</sub>·H<sub>2</sub>O)
*2 g magnesium sulphate heptahydrate (MgSO<sub>4</sub>·7H<sub>2</sub>O)
*1 g glucose
*1 g glucose
*1 L with distilled water
*1 L with distilled water

Latest revision as of 18:29, 3 September 2019

YMPD (Yeast Meat Peptone Dextrose)

For growth of Streptomyces griseus1.

Preparation

Per litre:

  • 2 g yeast extract
  • 2.2 g meat extract
  • 4 g Bacto peptone
  • 5 g NaCl
  • 2 g magnesium sulphate heptahydrate (MgSO4·7H2O)
  • 1 g glucose
  • 1 L with distilled water

Instructions

  1. Dissolve ingredients in water
  2. pH 7.2
  3. For solid medium, add 22 g agar
  4. Autoclave

Uses

Notes

References

1Ohnishi Y, et al. 2008. Genome Sequence of the Streptomycin-Producing Microorganism Streptomyces griseus IFO 13350. J. Bacteriol. 190: 4050-4060.