YMPD

YMPD (Yeast Meat Peptone Dextrose)

For growth of Streptomyces griseus¹.

Preparation

Per litre:

• 2 g yeast extract
• 2.2 g meat extract
• 4 g Bacto peptone
• 5 g NaCl
• 2 g magnesium sulphate heptahydrate (MgSO₄·7H₂O)
• 1 g glucose
• 1 L with distilled water

Instructions

1. Dissolve ingredients in water
2. pH 7.2
3. For solid medium, add 22 g agar
4. Autoclave

References

¹Ohnishi Y, et al. 2008. Genome Sequence of the Streptomycin-Producing Microorganism Streptomyces griseus IFO 13350. J. Bacteriol. 190: 4050-4060.