PSET152: Difference between revisions
From ActinoBase
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Key Components & Origin | |||
• Backbone: Derived from a standard E. coli plasmid vector (such as pBR322/pUC lineage) containing an E. coli replication origin (ori) and an origin of transfer (oriT) derived from a broad-host-range plasmid like RK2 to allow conjugal transfer. | |||
• Integrative Machinery: Incorporates the attachment site (attP) and the integrase gene (int) from the temperate actinophage φC31. | |||
• Selection Marker: Carries the aac(3)IV gene, which confers resistance to apramycin in both E. coli and Streptomyces. | |||
==Features== | ==Features== | ||
Revision as of 09:23, 10 October 2026
Key Components & Origin
• Backbone: Derived from a standard E. coli plasmid vector (such as pBR322/pUC lineage) containing an E. coli replication origin (ori) and an origin of transfer (oriT) derived from a broad-host-range plasmid like RK2 to allow conjugal transfer.
• Integrative Machinery: Incorporates the attachment site (attP) and the integrase gene (int) from the temperate actinophage φC31.
• Selection Marker: Carries the aac(3)IV gene, which confers resistance to apramycin in both E. coli and Streptomyces.
Features
Apramycin resistance marker allows for selection of the plasmid after conjkugation from E. coli
History
Map
