PSET152: Difference between revisions
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==Features== | ==Features== | ||
• Backbone: Derived from a standard E. coli plasmid vector ( | • Backbone: Derived from a standard E. coli plasmid vector (pBR322/pUC lineage) containing an E. coli replication origin (ori) and an origin of transfer (oriT) derived from broad-host-range plasmid RK2 to allow conjugal transfer. | ||
• Integrative Machinery: Incorporates the attachment site (attP) and the integrase gene (int) from the temperate actinophage φC31. | • Integrative Machinery: Incorporates the attachment site (attP) and the integrase gene (int) from the temperate actinophage φC31. Integrates into the φC31 att site on the Streptomyces genome. | ||
• Selection Marker: Carries the aac(3)IV gene, which confers resistance to apramycin in both E. coli and Streptomyces. | • Selection Marker: Carries the aac(3)IV gene, which confers resistance to apramycin in both E. coli and Streptomyces. | ||
Revision as of 09:26, 10 October 2026
Features
• Backbone: Derived from a standard E. coli plasmid vector (pBR322/pUC lineage) containing an E. coli replication origin (ori) and an origin of transfer (oriT) derived from broad-host-range plasmid RK2 to allow conjugal transfer.
• Integrative Machinery: Incorporates the attachment site (attP) and the integrase gene (int) from the temperate actinophage φC31. Integrates into the φC31 att site on the Streptomyces genome.
• Selection Marker: Carries the aac(3)IV gene, which confers resistance to apramycin in both E. coli and Streptomyces.
History
The pSET152 plasmid was constructed and published by Bierman et al. in 1992 as an E. coli–Streptomyces shuttle and integrative vector
Map
