Cloning, Transformation and Expression of Gene Clusters: Difference between revisions
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[[ | [[ET Conjugation]] | ||
[https://academic.oup.com/nar/article/24/3/536/2359981 High Efficiency 5 Min Transformation of Escherichia coli] | |||
[[Heterologous expression of gene clusters]] | [[Heterologous expression of gene clusters]] | ||
[[ | [[TAR cloning]] | ||
[[ | [[Tri-parental mating]] to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into ''Streptomyces''. |
Revision as of 14:27, 7 August 2019
High Efficiency 5 Min Transformation of Escherichia coli
Heterologous expression of gene clusters
Tri-parental mating to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into Streptomyces.