ISP4

ISP 4

International Streptomyces Project medium 4, also named inorganic salts-starch agar

Preparation

• 10 g Soluble Starch
• 1g K2HPO4 (anhydrous)
• 1 g MgSO₄ x 7H₂O
• 1 g NaCl
• 2 g (NH₄)₂SO₄
• 2 g CaCO₃
• 1 L dH₂
• 1 mL Trace Salts solution
  • 0.1 g FeSO₄ x 7H₂O
  • 0.1 g MnCl₂ x 4H₂O
  • 0.1 g ZnSO₄ x 7H₂O
  • 100 mL dH₂O
• 20 g agar

Instructions

Prepare as two separate solutions and mix before adding agar and autoclaving

Solution 1

1. Make a paste of the soluble starch with a small volume of cold dH₂O
2. Bring the paste up to a volume of 500 mL

Solution 2

1. Dissolve the remaining constituents and trace salts solution in 500 mL dH₂O
2. Adjust the pH so that it's between 7 and 7.4

Uses

• Screening for antibiotic production