PSS170: Difference between revisions
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{{DISPLAYTITLE: pSS170}} | {{DISPLAYTITLE: pSS170}} (aka pIJ10770) | ||
==Use== | ==Use== | ||
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==References== | ==References== | ||
pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323. | pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323. | ||
Schlimpert S, Wasserstrom S, Chandra G, Bibb MJ, Findlay KC, Flärdh K, et al. Two dynamin-like proteins stabilize FtsZ rings during Streptomyces sporulation. Proc Natl Acad Sci. 2017 Jul 25;114(30):E6176–83. | |||
Revision as of 10:45, 4 December 2025
(aka pIJ10770)
Use
pSS170 is used for introducing genes to Streptomyces. Because this plasmid is lacking a promoter upstream of the multiple cloning site (MCS), the gene of interest is often cloned in with its native promoter, in order to complement a knockout mutant
Features
Streptomyces phage phiBT1 integration site. Hygromycin resistance gene, oriT, ColEi origin of replication
History
Derived from pMS82 - https://www.addgene.org/68110/
Map
Sequence links
References
pMS82 background vector - Gregory, M.A., Till, R. and Smith, M.C., 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. Journal of bacteriology, 185(17), pp.5320-5323.
Schlimpert S, Wasserstrom S, Chandra G, Bibb MJ, Findlay KC, Flärdh K, et al. Two dynamin-like proteins stabilize FtsZ rings during Streptomyces sporulation. Proc Natl Acad Sci. 2017 Jul 25;114(30):E6176–83.
