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• 12:43, 18 July 2024 Matt Hutchings talk contribs created page Protein production in Streptomyces (Created page with """Protocol for "Streptomyces" protein expression"" ""Background"" Plasmid pIJ6021 allows for high level protein production in "Streptomyces" species. This plasmid was first described by Bibb et al in 19951 and utilises the pIJ101 origin of replication which leads to high copy number, sometimes approaching 100 copies per cell. It includes the thiostrepton inducible tipA promoter to induce expression of the target gene. However, pIJ6021 lacks an E. coli replicon, meanin...")