Difference between revisions of "CRISPR/Cas9-mediated genome editing"

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Can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.  
 
Can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.  
  
[[pCRISPomyces2]] [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459934/ Cobb et al 2014  4:723–728]
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[https://pubs.acs.org/doi/10.1021/acssynbio.5b00038 pCRISPR-Cas9]
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[[pCRISPomyces2]]

Revision as of 11:15, 5 August 2019

Can be used for precise CRISPR/Cas9-mediated genome engineering of Streptomyces strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.

pCRISPR-Cas9

pCRISPomyces2