Difference between revisions of "CRISPR/Cas9-mediated genome editing"

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CRISPR/Cas9 can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.  
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Can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.  
  
 
[[pCRISPomyces2]]
 
[[pCRISPomyces2]]

Revision as of 14:20, 19 July 2019

Can be used for precise CRISPR/Cas9-mediated genome engineering of Streptomyces strains. Mutations made have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. Several different plasmids have been constructed and published and are listed below. Click the links to find out more.

pCRISPomyces2