Difference between revisions of "Cloning, Transformation and Expression of Gene Clusters"

From ActinoBase
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[[TAR cloning]]
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[[ET Conjugation]]
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[https://academic.oup.com/nar/article/24/3/536/2359981 High Efficiency 5 Min Transformation of Escherichia coli]
  
 
[[Heterologous expression of gene clusters]]
 
[[Heterologous expression of gene clusters]]
  
[[Tri-parental mating]] to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into ''Streptomyces''.
+
[[TAR cloning]]
  
[[ET Conjugation]]
+
[[Tri-parental mating]] to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into ''Streptomyces''.

Revision as of 15:27, 7 August 2019

ET Conjugation

High Efficiency 5 Min Transformation of Escherichia coli

Heterologous expression of gene clusters

TAR cloning

Tri-parental mating to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into Streptomyces.