Difference between revisions of "Cloning, Transformation and Expression of Gene Clusters"

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[[Conjugation using ET12567/pUZ8002]]
 
[[Conjugation using ET12567/pUZ8002]]
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[[Heterologous expression of gene clusters]]
  
 
[https://academic.oup.com/nar/article/24/3/536/2359981 High Efficiency 5 Minute Transformation of Escherichia coli]
 
[https://academic.oup.com/nar/article/24/3/536/2359981 High Efficiency 5 Minute Transformation of Escherichia coli]
 
[[Heterologous expression of gene clusters]]
 
  
 
[[TAR cloning]]
 
[[TAR cloning]]
  
 
[[Tri-parental mating]] to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into ''Streptomyces''.
 
[[Tri-parental mating]] to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into ''Streptomyces''.

Revision as of 15:29, 7 August 2019

Conjugation using ET12567/pUZ8002

Heterologous expression of gene clusters

High Efficiency 5 Minute Transformation of Escherichia coli

TAR cloning

Tri-parental mating to generate ET12567 strains for the conjugation of large PAC clones or kanamycin marked vectors into Streptomyces.