Growing Marchantia from gemma spores: Difference between revisions
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{{DISPLAYTITLE: Growing Marchantia from gemma spores}} | {{DISPLAYTITLE: Growing '''Marchantia'' from gemma spores}} | ||
==Marchantia and their lifecycle== | ==''Marchantia'' and their lifecycle== | ||
Marchantia are a genus of plant in the | ''Marchantia'' (or liverworts) are a genus of plant in the bryophyte taxonomic division along with mosses and hornworts which are thought to be the oldest extant plants on earth. … | ||
<br /> | <br /> | ||
Marchantia polymorpha (The ‘Common liverwort’) is used as a model organism in biological studies. | ''Marchantia polymorpha'' (The ‘Common liverwort’) is used as a model organism in biological studies. | ||
==You will need:== | ==You will need:== | ||
*Healthy Marchantia plant with developed gemma cups and gemma | *Healthy ''Marchantia'' plant with developed gemma cups and gemma | ||
*dH<sub>2</sub>O | *dH<sub>2</sub>O | ||
*Sterile pipette tips of any size | *Sterile pipette tips of any size | ||
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==Protocol== | ==Protocol== | ||
#In a sterile environment, place a nylon mesh sheet onto your MS+B5 agar plates. | #In a sterile environment, place a nylon mesh sheet onto your MS+B5 agar plates. | ||
#Submerge the Marchantia plant in water and agitate gemma cups with a pipette tip so that the gemma float to the surface of the water. | #Submerge the ''Marchantia'' plant in water and agitate gemma cups with a pipette tip so that the gemma float to the surface of the water. | ||
#Pick up a single gemma spore with the tip of a pipette tip and transfer over to the nylon mesh plate. | #Pick up a single gemma spore with the tip of a pipette tip and transfer over to the nylon mesh plate. | ||
#Close the lid of the plate and seal with micropore tape. | #Close the lid of the plate and seal with micropore tape. | ||
Revision as of 13:26, 26 October 2021
Marchantia and their lifecycle
Marchantia (or liverworts) are a genus of plant in the bryophyte taxonomic division along with mosses and hornworts which are thought to be the oldest extant plants on earth. …
Marchantia polymorpha (The ‘Common liverwort’) is used as a model organism in biological studies.
You will need:
- Healthy Marchantia plant with developed gemma cups and gemma
- dH2O
- Sterile pipette tips of any size
- Sterile tweezers
- MS+B5 agar plates
- Sterile nylon mesh (cut to the shape of petri dish)
- Micropore tape
Protocol
- In a sterile environment, place a nylon mesh sheet onto your MS+B5 agar plates.
- Submerge the Marchantia plant in water and agitate gemma cups with a pipette tip so that the gemma float to the surface of the water.
- Pick up a single gemma spore with the tip of a pipette tip and transfer over to the nylon mesh plate.
- Close the lid of the plate and seal with micropore tape.
