ISP3

International Streptomyces Project-3 Medium (ISP-3)

International Streptomyces Project Medium 3 (ISP-3) is a rich general use media. Alternatively called oatmeal agar.

Preparation

• 20 g White Oats
• 18 g Agar
• 1 mL Trace salts solution
  • 0.1 g FeSO₄ x 7H₂O
  • 0.1 g MnCl₂ x 4H₂O
  • 0.1 g ZnSO₄ x 7H₂O
  • 100 mL dH₂O
• 1 L dH₂O

Instructions

1. Boil 20 g oats in 1L dH₂O for 20 mins
2. Filter oats through cheesecloth and top volume up to 1L
3. Adjust pH to 7.2, add agar, and autoclave
4. Add 1 mL Trace Salts solution

Uses

• Inducing sporulation of environmental isolates
• Screening for antibiotic production

Notes

When screening for antibiotic production, there can be significant differences between media prepared in-house and bought in media