Isolating Actinobacteria from soil environments: Difference between revisions
From ActinoBase
(Created page with "Isolating Actinobacteria from soil environments =Equipment & Materials= *Sterile containers to transport the soil *A heat block *Plates of Isolation media *A vortexer *¼ Str...") |
No edit summary |
||
Line 1: | Line 1: | ||
Isolating Actinobacteria from soil environments | =Isolating Actinobacteria from soil environments= | ||
=Equipment & Materials= | =Equipment & Materials= |
Latest revision as of 14:20, 25 October 2019
Isolating Actinobacteria from soil environments
Equipment & Materials
- Sterile containers to transport the soil
- A heat block
- Plates of Isolation media
- A vortexer
- ¼ Strength Ringer’s Solution
- Nalidixic acid, cycloheximide, and nystatin
Preparation of Soil
- Incubate 1g of soil at 50oC for 1 hour in order to kill vegetative organisms
- Suspend the soil in 10 mL ¼ Strength Ringer’s and vortex for 10 mins in order to thoroughly suspend your sample
- Dilute your soil suspension 1/10 and 1/100 and spread 200 µL of these onto plates of your isolation media supplemented with 25 µg mL-1 nystatin, 25 µg mL-1 cycloheximide, and 10 µg ml-1 nalidixic acid
- Incubate your plates at 30oC for three weeks, the temperature and duration will vary depending on what you are aiming to isolate
- Select actinobacterial colonies that have grown and patch them onto the same agar in order to isolate the strains