LB: Difference between revisions
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Revision as of 19:54, 20 June 2019
LB
Known as Lennox Broth or Lysogeny Broth
For 1 Litre:
- 5g Yeast Extract
- 10g NaCl
- 10g Tryptone
- Up to 1L dH2O
Instructions
- Add 5g Yeast Extract, 10g NaCl and 10g Tryptone to a beaker
- Add 800ml dH2O and stir until all solids are fully suspended
- Make up to 1L with dH2O
- For solid media, add 4g agar per 200ml LB
- Autoclave
Note When using Hygromycin, for example when cloning into pSS170, use the -NaCl (no salt) version of LB.
-NaCl LB
For 1 Litre:
- 5g Yeast Extract
- 10g NaCl
- 10g Tryptone
- Up to 1L dH2O