LB: Difference between revisions
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=LB= | =LB= | ||
Known as | Known as Lysogeny Broth | ||
==For 1 Litre:== | ==For 1 Litre:== | ||
| Line 15: | Line 15: | ||
#Autoclave | #Autoclave | ||
Notes: | |||
This is the Miller formulation, with 10g/L NaCl. Alternatively, 5g/L NaCl can be used (Lennox formulation) or no salt (see below). | |||
The -NaCl (no salt) version of LB should be used when using salt sensitive antibiotics like [[hygromycin]]: for example, when cloning into [[pSS170]]. (Alternatively, [[Difco Nutrient Agar]] can be used for hygromycin selection.) | |||
To make liquid LB medium, omit the agar and autoclave as above. | |||
==-NaCl LB== | ==-NaCl LB== | ||
===For 1 Litre:=== | ===For 1 Litre:=== | ||
*5g Yeast Extract | *5g Yeast Extract | ||
*10g Tryptone | *10g Tryptone | ||
*Up to 1L dH2O | *Up to 1L dH2O | ||
Revision as of 12:49, 25 June 2019
LB
Known as Lysogeny Broth
For 1 Litre:
- 5g Yeast Extract
- 10g NaCl
- 10g Tryptone
- Up to 1L dH2O
Instructions
- Add 5g Yeast Extract, 10g NaCl and 10g Tryptone to a beaker
- Add 800ml dH2O and stir until all solids are fully suspended
- Make up to 1L with dH2O
- For solid media, add 4g agar per 200ml LB
- Autoclave
Notes: This is the Miller formulation, with 10g/L NaCl. Alternatively, 5g/L NaCl can be used (Lennox formulation) or no salt (see below). The -NaCl (no salt) version of LB should be used when using salt sensitive antibiotics like hygromycin: for example, when cloning into pSS170. (Alternatively, Difco Nutrient Agar can be used for hygromycin selection.)
To make liquid LB medium, omit the agar and autoclave as above.
-NaCl LB
For 1 Litre:
- 5g Yeast Extract
- 10g Tryptone
- Up to 1L dH2O
