LB
From ActinoBase
LB
Known as Lysogeny Broth
For 1 Litre:
- 5g Yeast Extract
- 10g NaCl
- 10g Tryptone
- Up to 1L dH2O
Instructions
- Add 5g Yeast Extract, 10g NaCl and 10g Tryptone to a beaker
- Add 800ml dH2O and stir until all solids are fully suspended
- Make up to 1L with dH2O
- For solid media, add 4g agar per 200ml LB
- Autoclave
Notes: This is the Miller formulation, with 10g/L NaCl. Alternatively, 5g/L NaCl can be used (Lennox formulation) or no salt (see below). The -NaCl (no salt) version of LB should be used when using salt sensitive antibiotics like hygromycin: for example, when cloning into pSS170. (Alternatively, Difco Nutrient Agar can be used for hygromycin selection.)
To make liquid LB medium, omit the agar and autoclave as above.
-NaCl LB
For 1 Litre:
- 5g Yeast Extract
- 10g Tryptone
- Up to 1L dH2O