L buffer: Difference between revisions
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(Created page with "'''L (lysis) buffer''' (Thompson ''et al.'' 1982) Mix the following sterile solutions: *100 ml Sucrose (10.3%) *10 ml TES buffer (5.73%, adjusted to pH7.2) *1 ml K<sub>2</su...") |
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*0.2 ml [[Trace element solution]] | *0.2 ml [[Trace element solution]] | ||
*1 ml KH<sub>2</sub>PO<sub>4</sub> (0.5%) | *1 ml KH<sub>2</sub>PO<sub>4</sub> (0.5%) | ||
*0.1 ml MgCl<sub>2</sub>.6H<sub>2</sub>O (2.5M) | *0.1 ml MgCl<sub>2</sub> . 6H<sub>2</sub>O (2.5M) | ||
*1 ml CaCl<sub>2</sub> (0.25M) | *1 ml CaCl<sub>2</sub> (0.25M) | ||
This stock solution keeps indefinitely. Just before use dissolve lysozyme in a sample of the solution at a concentration of 1 mg/ml and sterilise by filtration. | This stock solution keeps indefinitely. Just before use dissolve lysozyme in a sample of the solution at a concentration of 1 mg/ml and sterilise by filtration. | ||
Latest revision as of 13:21, 4 September 2019
L (lysis) buffer (Thompson et al. 1982)
Mix the following sterile solutions:
- 100 ml Sucrose (10.3%)
- 10 ml TES buffer (5.73%, adjusted to pH7.2)
- 1 ml K2SO4 (2.5%)
- 0.2 ml Trace element solution
- 1 ml KH2PO4 (0.5%)
- 0.1 ml MgCl2 . 6H2O (2.5M)
- 1 ml CaCl2 (0.25M)
This stock solution keeps indefinitely. Just before use dissolve lysozyme in a sample of the solution at a concentration of 1 mg/ml and sterilise by filtration.
