L buffer
From ActinoBase
L (lysis) buffer (Thompson et al. 1982)
Mix the following sterile solutions:
- 100 ml Sucrose (10.3%)
- 10 ml TES buffer (5.73%, adjusted to pH7.2)
- 1 ml K2SO4 (2.5%)
- 0.2 ml Trace element solution
- 1 ml KH2PO4 (0.5%)
- 0.1 ml MgCl2.6H2O (2.5M)
- 1 ml CaCl2 (0.25M)
This stock solution keeps indefinitely. Just before use dissolve lysozyme in a sample of the solution at a concentration of 1 mg/ml and sterilise by filtration.