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Revision as of 11:50, 26 June 2019
MM Medium
Preparation
Per litre:
- 0.5g L-asparagine
- 0.5g K2HPO4
- 0.2g MgSO4*7H2O
- 0.01g FeSO4*7H2O
- 10g glucose (added after autoclaving)
- 10g agar
- =1L dH2O
Instructions
- Dissolve all of the ingredients except the agar and the glucose in the distilled water
- Adjust the pH to 7.0-7.2
- Dispense 200ml into flasks each containing 2g agar
- Autoclave
- After autoclaving, add 4 ml sterile 50% glucose solution to each flask
- Add any other growth factors required for auxotrophic mutants
Uses
Notes
References
Hopwood DA. Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol Rev. 1967;31(4):373–403.