MS+B5

Preparation

Per Litre:

• 2.25 g g Murashige & Skoog Medium Without Vitamins
• 0.056 g Gamborg B5 Vitamin Mixture
• 12 g Agar
• 1 L tap water

Instructions

1. Dissolve 2.25g Murashige & Skoog and 0.056g Gamborg B5 in 1L tap water
2. Add 1.2g agar to each 250mL flask
3. Add 100mL solution to each flask
4. Autoclave

Uses

• Routine growth of Marchantia plants
• Growing Marchantia from gemma spores