Metabolite extractions

HP-20 resin Ethyl Acetate (EtOAc) extractions from liquid cultures

HP-20 resin and Media Preparation

HP-20 resin activation: Cover 100 g of dried HP-20 resin with approximately 300 mL of EtOAc in a clean glass bottle with polypropylene lid and shake for 10 minutes. Filter the HP-20 resin using a Buchnar funnel and filter paper and discard the EtOAc into an appropriate flammable solvent waste container. Add HP-20 resin back into the used bottle and cover again with 300 mL ethyl acetate.

Adding HP-20 resin to media: Dispense 50 mL of media into 250 mL conical flasks. Use a Buchner funnel and filter paper to weigh out 5% w/v of HP-20 resin (2.5 g of HP-20 resin per 50 mL of media).

Notes: the HP-20 resin should be completely dry. Never contact plastic with EtOAc or HP-20 resin that has been stored in EtOAc. Add HP-20 resin to fermentation media prior to autoclaving.