MtrA methods are here

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This page contains methods used in the following study:

Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces venezuelae. Manuscript in preparation.

Microbiology methods (links to sub pages)

SFM agar recipe


Streptomyces spore preparation


The PCRISPomyces-2 plasmid for CRISPR/Cas9 mediated genome editing


ReDCaT surface plasmon resonance

This technique is described in this publication [[1]]. Methods are given in detail below.