PCRISPomyces-2: Difference between revisions
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This plasmid can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. To our knowledge it has been successfully used in [https://doi.org/10.1128/mSphere.00305-16 S. albidoflavus], S. coelicolor, [https://pubs.rsc.org/en/content/articlelanding/2017/SC/C6SC04265A#!divAbstract S. formicae], [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459934/ S. lividans] and S. venezuelae. Mutations have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. | This plasmid can be used for precise CRISPR/Cas9-mediated genome engineering of <em>Streptomyces</em> strains. To our knowledge it has been successfully used in [https://doi.org/10.1128/mSphere.00305-16 S. albidoflavus], S. coelicolor, [https://pubs.rsc.org/en/content/articlelanding/2017/SC/C6SC04265A#!divAbstract S. formicae], [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459934/ S. lividans] and S. venezuelae. Mutations have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci. | ||
[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459934/ The paper describing pCRISPomyces2] | |||
[ | The protocol from Matt Hutchings lab - [http://www.hutchingslab.uk/downloads/pCRISPOmyces2.pdf] download as a PDF. | ||
The plasmid is available from AddGene under MTA: [https://www.addgene.org/61737/ click here] | The plasmid is available from AddGene under MTA: [https://www.addgene.org/61737/ click here] | ||
[[file:CRISPR.png]] | [[file:CRISPR.png]] | ||
Revision as of 20:15, 18 July 2019
This plasmid can be used for precise CRISPR/Cas9-mediated genome engineering of Streptomyces strains. To our knowledge it has been successfully used in S. albidoflavus, S. coelicolor, S. formicae, S. lividans and S. venezuelae. Mutations have ranged from 1-100kbp deletions, precise codon changes to alter amino acids and insertions to add Flag-tags to proteins encoded at their native loci.
The paper describing pCRISPomyces2
The protocol from Matt Hutchings lab - [1] download as a PDF.
The plasmid is available from AddGene under MTA: click here
