PIJ10257: Difference between revisions
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==Use== | ==Use== | ||
This vector can be used to introduce a gene or set of genes | This vector (originally produced and published by the Buttner group <sup>1</sup>) can be used to introduce a gene or set of genes into <em> Streptomyces </em> under the control of the constitutive <em> ermE*</em> promoter <sup>2</sup>. This can be useful for over expression or complementation experiments. | ||
==Features== | ==Features== | ||
* Hygromycin resistance gene | * Hygromycin resistance gene | ||
* <em>ermE*</em> promoter | * <em>ermE*</em> promoter<sup>2</sup> | ||
* ΦBT1 phage integration site | * ΦBT1 phage integration site | ||
*Origin of transfer <em>oriT</em> from RK2 | |||
==History== | ==History== | ||
Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing <em> ermE*</em>, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ10257<sup>1</sup> | Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing <em> ermE*</em>, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ10257<sup>1</sup>. | ||
==Map== | ==Map== | ||
[[File: PIJ10257 Map2.png]] | |||
==Sequence links== | ==Sequence links== | ||
File available with annotations (john.t.munnoch@strath.ac.uk) pre-editing of configs for file upload. | |||
==Sequence== | |||
>pIJ10257_sequenced_by_genscript | |||
GATCCGCGGCCGCGCGCGATATCGAATTCGTAATCATGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTGGTTCATGTGCAGCTCCATCAGCAAAAGGGGATGATAAGTTTATCACCACCGACTATTTGCAACAGTGCCGTTGATCGTGCTATGATCGACTGATGTCATCAGCGGTGGAGTGCAATGTCGTGCAATACGAATGGCGAAAAGCCGAGAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGGGTTGCCGAGCTGGGATGGAAGCTCGGCCGACCACCCTGGAGGAGATGATCGAGGATGCCAGGGCCTTTCACGCCCGCCGCTGCTGAGCGTCCGCCGCCGGGCCCGCACCGCCGTCGGCCGGCCCGCTCCGGGCTCGCAGCAGCGGGCTTCGGCGCGGGCCCGGGGCTCCCGAGCGCGGGCGGGGCTCCGGGCGGCCGCCGGGGGCCGGGGGCGGCGCCGGGCGGCCCGGGGCGTCAGGCGCCGGGGGCGGTGTCCGGCGGCCCCCAGAGGAACTGCGCCAGTTCCTCCGGATCGGTGAAGCCGGAGAGATCCAGCGGGGTCTCCTCGAACACCTCGAAGTCGTGCAGGAAGGTGAAGGCGAGCAGTTCGCGGGCGAAGTCCTCGGTCCGCTTCCACTGCGCCCCGTCGAGCAGCGCGGCCAGGATCTCGCGGTCGCCCCGGAAGGCGTTGAGATGCAGTTGCACCAGGCTGTAGCGGGAGTCTCCCGCATAGACGTCGGTGAAGTCGACGATCCCGGTGACCTCGGTCGCGGCCAGGTCCACGAAGATGTTGGTCCCGTGCAGGTCGCCGTGGACGAACCGGGGTTCGCGGCCGGCCAGCAGCGTGTCCACGTCCGGCAGCCAGTCCTCCAGGCGGTCCAGCAGCCGGGGCGAGAGGTAGCCCCACCCGCGGTGGTCCTCGACGGTCGCCGCGCGGCGTTCCCGCAGCAGTTCCGGGAAGACCTCGGAATGGGGGGTGAGCACGGTGTTCCCGGTCAGCGGCACCCTGTGCAGCCGGCCGAGCACCCGGCCGAGTTCGCGGGCCAGGGCGAGCAGCGCGTTCCGGTCGGTCGTGCCGTCCATCGCGGACCGCCAGGTGGTGCCGGTCATCCGGCTCATCACCAGGTAGGGCCACGGCCAGGCTCCGGTGCCGGGCCGCAGCTCGCCGCGGCCGAGGAGGCGGGGCACCGGCACCGGGGCGTCCGCCAGGACCGCGTACGCCTCCGACTCCGACGCGAGGCTCTCCGGACCGCACCAGTGCTCGCCGAACAGCTTGATCACCGGGTCGGGCTCGCCGACCAGTACGGGGTTGGTGCTCTCGCCGGGCACCCGCAGCACCGGCGGCACCGGCAGCCCGAGCTCCTCCAGGGCTCGGCGGGCCAGCGGCTCCCAGAATTCCTGGTCGTTCCGCAGGCTCGCGTAGGAATCATCCGAATCAATACGGTCGAGAAGTAACAGGGATTCTTGTGTCACAGCGGACCTCTATTCACAGGGTACGGGCCGGCTTAATTCCGCACGGCCGGTCGCGACACGGCCTGTCCGCACCGCGGTCAGGCGTTGACGATGACGGGCTGGTCGGCCACGTCGGGGACGTTCTCGGTGGTGCTGCGGTCGGGATCGCCAATCTCTACGGGCCGACCGAGGCGACGGTGTACGCCACCGCCTGGTTCTGCGACGGCGAGGCGCCGCCAGGCCCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTNAGGTACCAGCCCGACCCGAGCACGCGCCGGCACGCCTGGTCGATGTCGGACCGGAGTTCGAGGTACGCGGCTTGCAGGTCCAGGAAGGGGACGTCCATGCGAGTGTCCGTTCGAGTGGCGGCTTGCGCCCGATGCTAGTCGCGGTTGATCGGCGATCGCAGGTGCACGCGGTCGATCTTGACGGCTGGCGAGAGGTGCGGGGAGGATCTGACCGACGCGGTCCACACGTGGCACCGCGATGCTGTTGTGGGCACAATCGTGCCGGTTGGTAGGATCGTCTAGAACAGGAGGCCCCATATGAGATCTCGAGTGATTAATTAACTAGAGTCGACCTGCATAGATCtaagcttggatccTAGGTTCTCATGAGCGGAGAACGAGATGACGTTGGAGGGGCAAGGTCGCGCTGATTGCTGGGGCAACACGTGGAGCGGATCGGGGATTGTCTTTCTTCAGCTCGCTGATGATATGCTGACGCTCAATGCCGTTTGGCCTCCGACTAACGAAAATCCCGCATTTGGACGGCTGATCCGATTGGCACGGCGGACGGCGAATGGCGGAGCAGACGCTCGTCCGGGGGCAATGAGATATGAAAAAGCCTGAACTCACCGCGACGTATCGGGCCCTGGCCAGCTAGCTAGAGTCGACCTGCAGGTCCCCGGGGATCGGTCTTGCCTTGCTCGTCGGTGATGTACTTCACCAGCTCCGCGAAGTCGCTCTTCTTGATGGAGCGCATGGGGACGTGCTTGGCAATCACGCGCACCCCCCGGCCGTTTTAGCGGCTAAAAAAGTCATGGCTCTGCCCTCGGGCGGACCACGCCCATCATGACCTTGCCAAGCTCGTCCTGCTTCTCTTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCCGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGATCCGTCGACCTGCAGGCATGCTGGCGCCGGACGGGGCTTCAGACGTTTCGGGTGCTGGGTTGTTGTCTCTGGACAGTGATCCATGGGAAACTACTCAGCACCACCAATGTTCCCAAAAGAAAGCGCAGGTCAGCGCCCATGAGCCAAGATCTAGGCATGTCGCCCTTCATCGCTCCCGACGTCCCTGAGCACCTTCTAGACACTGTTCGCGTCTTCCTGTACGCGCGTCAGTCTAAGGGCCGGTCCGACGGCTCAGACGTGTCGACCGAAGCACAGCTAGCGGCCGGTCGTGCGTTGGTCGCGTCTCGCAACGCCCAGGGGGGTGCGCGCTGGGTCGTGGCAGGTGAGTTCGTGGACGTCGGGCGCTCCGGCTGGGACCCGAACGTGACCCGTGCCGACTTCGAGCGCATGATGGGCGAAGTCCGCGCCGGCGAAGGTGACGTTGTCGTTGTGAATGAGCTTTCCCGGCTCACTCGCAAGGGCGCCCATGACGCGCTCGAAATCGACAACGAATTGAAGAAGCACGGCGTGCGCTTCATGTCGGTTCTTGAGCCGTTCCTTGACACGTCTACCCCTATCGGCGTCGCCATTTTCGCGCTGATCGCTGCCCTTGCGAAACAGGACAGTGACCTGAAGGCGGAGCGCCTGAAGGGTGCGAAAGACGAGATTGCCGCGCTGGGTGGCGTTCACTCGTCTTCCGCCCCGTTCGGAATGCGCGCCGTGCGCAAGAAGGTCGATAATCTCGTGATCTCCGTTCTTGAGCCGGACGAAGACAACCCGGATCACGTCGAGCTAGTTGAGCGCATGGCGAAAATGTCGTTCGAAGGCGTGTCCGACAACGCCATTGCAACGACCTTCGAGAAGGAAAAGATCCCGTCGCCCGGAATGGCTGAGAGACGCGCCACGGAAAAGCGTCTTGCGTCCATCAAGGCACGTCGCCTGAACGGCGCTGAAAAGCCGATCATGTGGCGCGCTCAAACGGTCCGATGGATTCTCAACCATCCCGCAATCGGCGGTTTCGCATTCGAGCGTGTGAAGCACGGTAAGGCGCACATCAACGTCATACGGCGCGACCCCGGCGGCAAGCCGCTAACGCCCCACACGGGCATTCTCAGCGGCTCGAAGTGGCTTGAGCTTCAAGAGAAGCGTTCCGGGAAGAATCTCAGCGACCGGAAGCCTGGGGCCGAAGTCGAACCGACGCTTCTGAGCGGGTGGCGTTTCCTGGGGTGCCGAATCTGCGGCGGCTCAATGGGTCAGTCCCAGGGTGGCCGTAAGCGCAACGGCGACCTTGCCGAAGGCAATTACATGTGCGCCAACCCGAAGGGGCACGGCGGCTTGTCGGTCAAGCGCAGCGAACTGGACGAATTCGTTGCTTCGAAGGTGTGGGCACGGCTCCGCACAGCCGACATGGAAGATGAACACGATCAGGCATGGATTGCCGCCGCTGCGGAGCGCTTCGCCCTTCAGCACGACCTAGCGGGGGTGGCCGATGAGCGGCGCGAACAACAGGCGCACCTAGACAACGTGCGGCGCTCCATCAAGGACCTTCAGGCGGACCGTAAGGCCGGTCTGTACGTCGGGCGTGAAGAGCTGGAAACGTGGCGCTCAACGGTGCTGCAATACCGGTCCTACGAAGCGGAGTGCACGACCCGACTCGCTGAGCTTGACGAGAAGATGAACGGCAGCACCCGCGTTCCGTCTGAGTGGTTCAGCGGCGAAGACCCGACGGCCGAAGGGGGCATCTGGGCAAGCTGGGACGTGTACGAGCGTCGGGAGTTCCTGAGCTTCTTCCTTGACTCCGTCATGGTCGACCGGGGGCGCCACCCTGAGACGAAGAAATACATCCCCCTGAAGGACCGTGTGACGCTCAAGTGGGCGGAGCTGCTGAAGGAGGAAGACGAAGCGAGCGAAGCCACTGAGCGGGAGCTTGCGGCGCTGTAGCGCACAGCGGGAGGGGTCGAGCCGGCGGACGGTTCGGCCCCTTTTTTGGCCTTGAAATCGTTAGTTAGGCTAACTAGTAGTTCCTTCGTCACCATAGCGGGCAGGGAGCG | |||
==References== | ==References== | ||
# Hong, H., Hutchings, M. I., Hill, L. M., Buttner, M. J. (2005). The Role of the Novel Fem Protein VanK in Vancomycin Resistance in Streptomyces coelicolor. ''Journal of Biological Chemistry'', 280, pp. 13055-13061. doi: 10.1074/jbc.M413801200 | # Hong, H., Hutchings, M. I., Hill, L. M., Buttner, M. J. (2005). The Role of the Novel Fem Protein VanK in Vancomycin Resistance in Streptomyces coelicolor. ''Journal of Biological Chemistry'', 280, pp. 13055-13061. doi: 10.1074/jbc.M413801200 | ||
# Bibb, M. J., Janssen, G. R., and Ward, J. M. (1985). Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. ‘’Gene’’, 38, pp. 215-226. doi: 10.1016/0378-1119(85)90220-3 | |||
Latest revision as of 14:24, 25 October 2019
Use
This vector (originally produced and published by the Buttner group 1) can be used to introduce a gene or set of genes into Streptomyces under the control of the constitutive ermE* promoter 2. This can be useful for over expression or complementation experiments.
Features
- Hygromycin resistance gene
- ermE* promoter2
- ΦBT1 phage integration site
- Origin of transfer oriT from RK2
History
Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing ermE*, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ102571.
Map
Sequence links
File available with annotations (john.t.munnoch@strath.ac.uk) pre-editing of configs for file upload.
Sequence
>pIJ10257_sequenced_by_genscript GATCCGCGGCCGCGCGCGATATCGAATTCGTAATCATGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTGGTTCATGTGCAGCTCCATCAGCAAAAGGGGATGATAAGTTTATCACCACCGACTATTTGCAACAGTGCCGTTGATCGTGCTATGATCGACTGATGTCATCAGCGGTGGAGTGCAATGTCGTGCAATACGAATGGCGAAAAGCCGAGAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGGGTTGCCGAGCTGGGATGGAAGCTCGGCCGACCACCCTGGAGGAGATGATCGAGGATGCCAGGGCCTTTCACGCCCGCCGCTGCTGAGCGTCCGCCGCCGGGCCCGCACCGCCGTCGGCCGGCCCGCTCCGGGCTCGCAGCAGCGGGCTTCGGCGCGGGCCCGGGGCTCCCGAGCGCGGGCGGGGCTCCGGGCGGCCGCCGGGGGCCGGGGGCGGCGCCGGGCGGCCCGGGGCGTCAGGCGCCGGGGGCGGTGTCCGGCGGCCCCCAGAGGAACTGCGCCAGTTCCTCCGGATCGGTGAAGCCGGAGAGATCCAGCGGGGTCTCCTCGAACACCTCGAAGTCGTGCAGGAAGGTGAAGGCGAGCAGTTCGCGGGCGAAGTCCTCGGTCCGCTTCCACTGCGCCCCGTCGAGCAGCGCGGCCAGGATCTCGCGGTCGCCCCGGAAGGCGTTGAGATGCAGTTGCACCAGGCTGTAGCGGGAGTCTCCCGCATAGACGTCGGTGAAGTCGACGATCCCGGTGACCTCGGTCGCGGCCAGGTCCACGAAGATGTTGGTCCCGTGCAGGTCGCCGTGGACGAACCGGGGTTCGCGGCCGGCCAGCAGCGTGTCCACGTCCGGCAGCCAGTCCTCCAGGCGGTCCAGCAGCCGGGGCGAGAGGTAGCCCCACCCGCGGTGGTCCTCGACGGTCGCCGCGCGGCGTTCCCGCAGCAGTTCCGGGAAGACCTCGGAATGGGGGGTGAGCACGGTGTTCCCGGTCAGCGGCACCCTGTGCAGCCGGCCGAGCACCCGGCCGAGTTCGCGGGCCAGGGCGAGCAGCGCGTTCCGGTCGGTCGTGCCGTCCATCGCGGACCGCCAGGTGGTGCCGGTCATCCGGCTCATCACCAGGTAGGGCCACGGCCAGGCTCCGGTGCCGGGCCGCAGCTCGCCGCGGCCGAGGAGGCGGGGCACCGGCACCGGGGCGTCCGCCAGGACCGCGTACGCCTCCGACTCCGACGCGAGGCTCTCCGGACCGCACCAGTGCTCGCCGAACAGCTTGATCACCGGGTCGGGCTCGCCGACCAGTACGGGGTTGGTGCTCTCGCCGGGCACCCGCAGCACCGGCGGCACCGGCAGCCCGAGCTCCTCCAGGGCTCGGCGGGCCAGCGGCTCCCAGAATTCCTGGTCGTTCCGCAGGCTCGCGTAGGAATCATCCGAATCAATACGGTCGAGAAGTAACAGGGATTCTTGTGTCACAGCGGACCTCTATTCACAGGGTACGGGCCGGCTTAATTCCGCACGGCCGGTCGCGACACGGCCTGTCCGCACCGCGGTCAGGCGTTGACGATGACGGGCTGGTCGGCCACGTCGGGGACGTTCTCGGTGGTGCTGCGGTCGGGATCGCCAATCTCTACGGGCCGACCGAGGCGACGGTGTACGCCACCGCCTGGTTCTGCGACGGCGAGGCGCCGCCAGGCCCCGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTNAGGTACCAGCCCGACCCGAGCACGCGCCGGCACGCCTGGTCGATGTCGGACCGGAGTTCGAGGTACGCGGCTTGCAGGTCCAGGAAGGGGACGTCCATGCGAGTGTCCGTTCGAGTGGCGGCTTGCGCCCGATGCTAGTCGCGGTTGATCGGCGATCGCAGGTGCACGCGGTCGATCTTGACGGCTGGCGAGAGGTGCGGGGAGGATCTGACCGACGCGGTCCACACGTGGCACCGCGATGCTGTTGTGGGCACAATCGTGCCGGTTGGTAGGATCGTCTAGAACAGGAGGCCCCATATGAGATCTCGAGTGATTAATTAACTAGAGTCGACCTGCATAGATCtaagcttggatccTAGGTTCTCATGAGCGGAGAACGAGATGACGTTGGAGGGGCAAGGTCGCGCTGATTGCTGGGGCAACACGTGGAGCGGATCGGGGATTGTCTTTCTTCAGCTCGCTGATGATATGCTGACGCTCAATGCCGTTTGGCCTCCGACTAACGAAAATCCCGCATTTGGACGGCTGATCCGATTGGCACGGCGGACGGCGAATGGCGGAGCAGACGCTCGTCCGGGGGCAATGAGATATGAAAAAGCCTGAACTCACCGCGACGTATCGGGCCCTGGCCAGCTAGCTAGAGTCGACCTGCAGGTCCCCGGGGATCGGTCTTGCCTTGCTCGTCGGTGATGTACTTCACCAGCTCCGCGAAGTCGCTCTTCTTGATGGAGCGCATGGGGACGTGCTTGGCAATCACGCGCACCCCCCGGCCGTTTTAGCGGCTAAAAAAGTCATGGCTCTGCCCTCGGGCGGACCACGCCCATCATGACCTTGCCAAGCTCGTCCTGCTTCTCTTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCCGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGATCCGTCGACCTGCAGGCATGCTGGCGCCGGACGGGGCTTCAGACGTTTCGGGTGCTGGGTTGTTGTCTCTGGACAGTGATCCATGGGAAACTACTCAGCACCACCAATGTTCCCAAAAGAAAGCGCAGGTCAGCGCCCATGAGCCAAGATCTAGGCATGTCGCCCTTCATCGCTCCCGACGTCCCTGAGCACCTTCTAGACACTGTTCGCGTCTTCCTGTACGCGCGTCAGTCTAAGGGCCGGTCCGACGGCTCAGACGTGTCGACCGAAGCACAGCTAGCGGCCGGTCGTGCGTTGGTCGCGTCTCGCAACGCCCAGGGGGGTGCGCGCTGGGTCGTGGCAGGTGAGTTCGTGGACGTCGGGCGCTCCGGCTGGGACCCGAACGTGACCCGTGCCGACTTCGAGCGCATGATGGGCGAAGTCCGCGCCGGCGAAGGTGACGTTGTCGTTGTGAATGAGCTTTCCCGGCTCACTCGCAAGGGCGCCCATGACGCGCTCGAAATCGACAACGAATTGAAGAAGCACGGCGTGCGCTTCATGTCGGTTCTTGAGCCGTTCCTTGACACGTCTACCCCTATCGGCGTCGCCATTTTCGCGCTGATCGCTGCCCTTGCGAAACAGGACAGTGACCTGAAGGCGGAGCGCCTGAAGGGTGCGAAAGACGAGATTGCCGCGCTGGGTGGCGTTCACTCGTCTTCCGCCCCGTTCGGAATGCGCGCCGTGCGCAAGAAGGTCGATAATCTCGTGATCTCCGTTCTTGAGCCGGACGAAGACAACCCGGATCACGTCGAGCTAGTTGAGCGCATGGCGAAAATGTCGTTCGAAGGCGTGTCCGACAACGCCATTGCAACGACCTTCGAGAAGGAAAAGATCCCGTCGCCCGGAATGGCTGAGAGACGCGCCACGGAAAAGCGTCTTGCGTCCATCAAGGCACGTCGCCTGAACGGCGCTGAAAAGCCGATCATGTGGCGCGCTCAAACGGTCCGATGGATTCTCAACCATCCCGCAATCGGCGGTTTCGCATTCGAGCGTGTGAAGCACGGTAAGGCGCACATCAACGTCATACGGCGCGACCCCGGCGGCAAGCCGCTAACGCCCCACACGGGCATTCTCAGCGGCTCGAAGTGGCTTGAGCTTCAAGAGAAGCGTTCCGGGAAGAATCTCAGCGACCGGAAGCCTGGGGCCGAAGTCGAACCGACGCTTCTGAGCGGGTGGCGTTTCCTGGGGTGCCGAATCTGCGGCGGCTCAATGGGTCAGTCCCAGGGTGGCCGTAAGCGCAACGGCGACCTTGCCGAAGGCAATTACATGTGCGCCAACCCGAAGGGGCACGGCGGCTTGTCGGTCAAGCGCAGCGAACTGGACGAATTCGTTGCTTCGAAGGTGTGGGCACGGCTCCGCACAGCCGACATGGAAGATGAACACGATCAGGCATGGATTGCCGCCGCTGCGGAGCGCTTCGCCCTTCAGCACGACCTAGCGGGGGTGGCCGATGAGCGGCGCGAACAACAGGCGCACCTAGACAACGTGCGGCGCTCCATCAAGGACCTTCAGGCGGACCGTAAGGCCGGTCTGTACGTCGGGCGTGAAGAGCTGGAAACGTGGCGCTCAACGGTGCTGCAATACCGGTCCTACGAAGCGGAGTGCACGACCCGACTCGCTGAGCTTGACGAGAAGATGAACGGCAGCACCCGCGTTCCGTCTGAGTGGTTCAGCGGCGAAGACCCGACGGCCGAAGGGGGCATCTGGGCAAGCTGGGACGTGTACGAGCGTCGGGAGTTCCTGAGCTTCTTCCTTGACTCCGTCATGGTCGACCGGGGGCGCCACCCTGAGACGAAGAAATACATCCCCCTGAAGGACCGTGTGACGCTCAAGTGGGCGGAGCTGCTGAAGGAGGAAGACGAAGCGAGCGAAGCCACTGAGCGGGAGCTTGCGGCGCTGTAGCGCACAGCGGGAGGGGTCGAGCCGGCGGACGGTTCGGCCCCTTTTTTGGCCTTGAAATCGTTAGTTAGGCTAACTAGTAGTTCCTTCGTCACCATAGCGGGCAGGGAGCG
References
- Hong, H., Hutchings, M. I., Hill, L. M., Buttner, M. J. (2005). The Role of the Novel Fem Protein VanK in Vancomycin Resistance in Streptomyces coelicolor. Journal of Biological Chemistry, 280, pp. 13055-13061. doi: 10.1074/jbc.M413801200
- Bibb, M. J., Janssen, G. R., and Ward, J. M. (1985). Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. ‘’Gene’’, 38, pp. 215-226. doi: 10.1016/0378-1119(85)90220-3
