PIJ10257: Difference between revisions
From ActinoBase
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==History== | ==History== | ||
Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing <em> ermE*</em>, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ10257. | Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing <em> ermE*</em>, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ10257<sup>1</sup>.. | ||
==Map== | ==Map== | ||
Revision as of 17:08, 8 August 2019
Use
This vector can be used to introduce a gene or set of genes from E. coli into Streptomyces , to be put under the control of the constitutive ermE* promoter. This can be useful for over expression or complementation experiments.
Features
- Hygromycin resistance gene
- ermE* promoter
- ΦBT1 phage integration site
History
Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing ermE*, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ102571..
Map
Sequence links
References
- Hong, H., Hutchings, M. I., Hill, L. M., Buttner, M. J. (2005). The Role of the Novel Fem Protein VanK in Vancomycin Resistance in Streptomyces coelicolor. Journal of Biological Chemistry, 280, pp. 13055-13061. doi: 10.1074/jbc.M413801200
